ABSTRACT
Objective :To investigate the effects of ethanol precipitate(ET-Pre) and RNA of Grifola frondosa on non-specific immunity in mouse.Methods:The common biological methods were used to examine the levels of cytokines and immunocyte activity. Results: The killing activity of the NK cells, the phagocytosis function of macrophages and the levels of TNF?/IL-1 in the animals treated with ET-Pre and RNA respectively were significantly higher than those in the control.The RNA was stronger than ET-Pre in increasing killing activity of NK cells and phagocytosis function of macrophages. Conclusion:Both ET-Pre and RNA extracts of Grifola frondosa may promote immunoactivity nonspecifi-cally and inhibit tumor cells indirectly.
ABSTRACT
Objective:To investigate the effect of Glycyrrhizin on the cytokines derived from peritoneal macrophages in mice.Methods:Glycyrrhizin was intraperitoneally administered 24 hr before the peritoneal macrophages (PMs) were harvested.The harvested PMs were then stimulated in vitro with lipopolysaccharide (LPS).The levels of tumor necrosis factor (TNF)-?,interleukin-12 (IL-12) p70,interleukin-10 (IL-10) and macrophage colony-stimulating factor (M-CSF) from culture supernatants were measured by ELISA.Results:Glycyrrhizin suppressed LPS-induced TNF-? production and increased LPS-induced IL-12 p70 production by PMs significantly. The production of IL-10 and M-CSF by PMs were not effected by Glycyrrhizin pretreatment.Conclusion:These findings demonstrate the ability of Glycyrrhizin to suppress LPS-induced TNF-? poduction and to enhance IL-12 production by peritoneal macrophages.
ABSTRACT
Objective:To investigate the expression of aromatase of renal tissue in systemic lupus erythematosus(SLE)model mice.Methods:BALB/c mice were induced SLE with homologuous splenic cell activated with ConA after being ovariectomized,and in the same time administered different doses of benzestrofol.E2 in peripheral blood and renal tissue was detected by ELISA and the expression of mRNA of aromatase in renal tissue was detected by RT-PCR in the 4th,6th,8th and 10th weeks.Results:The level of E2 of peripheral blood and renal tissue of SLE model mice became higher as benzestrofol exogenously administered heightened.Compared with control mice,the level of E2 in SLE model mice increased,and the expression of mRNA of aromatase of renal tissue increased with E2 increment.Conclusion:E2 promotes development of SLE through regulating expression of aromatase mRNA.